cfx connect real time system (Bio-Rad)
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Cfx Connect Real Time System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx+connect+real+time+pcr+system/CFX+Connect+Real-Time+PCR+Detection+System+Firmware+Update/pmc13092056-119-26-30
Average 99 stars, based on 15572 article reviews
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Quantitative RT-PCR:Article Title: Histone lactylation modification promotes docetaxel resistance and tumor progression through CNN1-Mediated autophagy and cell cycle arrest in Castration-resistant prostate cancer. Article Snippet: RNA was converted to cDNA utilizing the SweScript All-in-One RT SuperMix (G3337, Servicebio, Wuhan, China). .. Real-time RT-PCR was conducted on a BioRad Article Title: miRNA Sequencing and Differential Analysis of Testis in 1-Year-Old and 2-Year-Old Kazakh Horses. Article Snippet: .. Quantitative RT-qPCR was conducted using a Article Title: TRIM7 inhibits rabies virus replication by promoting K48-linked ubiquitination and degradation of RABV-M Article Snippet: The extracted RNA was reverse-transcribed to complementary DNA using RT-qPCR Fast Master Mix (Vazyme, China). .. RT-qPCR was performed on a Real-time Polymerase Chain Reaction:Article Title: Histone lactylation modification promotes docetaxel resistance and tumor progression through CNN1-Mediated autophagy and cell cycle arrest in Castration-resistant prostate cancer. Article Snippet: RNA was converted to cDNA utilizing the SweScript All-in-One RT SuperMix (G3337, Servicebio, Wuhan, China). .. Real-time RT-PCR was conducted on a BioRad Article Title: Pulsed Electric Fields as an Effective Tool for Toxoplasma gondii Inactivation. Article Snippet: Thirty milligrams of mouse brain or heart were homogenized in 300 μL of lysis buffer (Promega, Madison, WI, USA) and 37 μL of Proteinase K (Promega, Madison, WI, USA) using plastic hand-pestle rotating plungers in an Eppendorf, and subsequently incubated at 70 ◦C for 30 min. DNA extraction was performed using a Maxwell 16 Lev Blood DNA Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions. .. Amplification and detection of T. gondii were performed using a Article Title: White Adipose Tissue as a Functional Target of Secondary Bile Acids in Rainbow Trout ( Oncorhynchus mykiss ) Article Snippet: Then, 2 μg of RNA were reverse transcribed into complementary DNA (cDNA) in a 20 μL reaction using the GoScriptTM Reverse Transcription Mix with Random Primers (Promega, Madison, WI, USA), according to the supplied protocol. .. Quantitative real-time PCR (RT-qPCR) was performed on a Article Title: Biomimetic flowing vessel-on-a-chip recruiting glycocalyx features for investigating dexmedetomidine function Article Snippet: Fluorescence imaging was performed using an inverted fluorescent microscope (Zeiss Axio Vert.A1, Germany) and a confocal microscope (Nikon A1, Japan). .. Quantitative real-time PCR was performed on a Article Title: miRNA Sequencing and Differential Analysis of Testis in 1-Year-Old and 2-Year-Old Kazakh Horses. Article Snippet: .. Quantitative RT-qPCR was conducted using a Article Title: Surveillance on California dairy farms reveals multiple possible sources of H5N1 influenza virus transmission. Article Snippet: Lysis buffer was then added to the pre-aliquoted samples in BSL3 and subsequent nucleic acid extraction was performed in the BSL2 lab (per approved biosafety protocols) using the NucleoMag RNA kit (Machery-Nagel) on an EpMotion 5075 (Eppendorf), with inclusion of a negative extraction control (PBS) in each extraction run. .. Viral detection required both sample replicates to each exhibit amplification (have CT values) using H5 HA-specific primers TATAGARGGAGGATGGCAGG and ACDGCCTCAAAYTGAGTGTT and probe FAM-AGGGGAGTGGKTACGCTGCRGAC- Black hole quencher, with the iTaq Universal Probes One-Step kit (Bio-Rad, Cat. No. 1725141) on a Article Title: TRIM7 inhibits rabies virus replication by promoting K48-linked ubiquitination and degradation of RABV-M Article Snippet: The extracted RNA was reverse-transcribed to complementary DNA using RT-qPCR Fast Master Mix (Vazyme, China). .. RT-qPCR was performed on a Article Title: Epigenetic Regulation of Immune Dysfunction in Chronic Prostatitis/Chronic Pelvic Pain Syndrome. Article Snippet: .. Total RNA was extracted from the VB3 cells with TRIzol reagent (Invitrogen) and reverse‐transcribed with qScript cDNA SuperMix (QuantaBio) following the manufacturers' protocols. qRT‐PCR reactions were prepared with PerfeCTa SYBR Green SuperMix (QuantaBio) and run on a SYBR Green Assay:Article Title: Histone lactylation modification promotes docetaxel resistance and tumor progression through CNN1-Mediated autophagy and cell cycle arrest in Castration-resistant prostate cancer. Article Snippet: RNA was converted to cDNA utilizing the SweScript All-in-One RT SuperMix (G3337, Servicebio, Wuhan, China). .. Real-time RT-PCR was conducted on a BioRad Article Title: Pulsed Electric Fields as an Effective Tool for Toxoplasma gondii Inactivation. Article Snippet: Thirty milligrams of mouse brain or heart were homogenized in 300 μL of lysis buffer (Promega, Madison, WI, USA) and 37 μL of Proteinase K (Promega, Madison, WI, USA) using plastic hand-pestle rotating plungers in an Eppendorf, and subsequently incubated at 70 ◦C for 30 min. DNA extraction was performed using a Maxwell 16 Lev Blood DNA Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions. .. Amplification and detection of T. gondii were performed using a Article Title: White Adipose Tissue as a Functional Target of Secondary Bile Acids in Rainbow Trout ( Oncorhynchus mykiss ) Article Snippet: Then, 2 μg of RNA were reverse transcribed into complementary DNA (cDNA) in a 20 μL reaction using the GoScriptTM Reverse Transcription Mix with Random Primers (Promega, Madison, WI, USA), according to the supplied protocol. .. Quantitative real-time PCR (RT-qPCR) was performed on a Article Title: TRIM7 inhibits rabies virus replication by promoting K48-linked ubiquitination and degradation of RABV-M Article Snippet: The extracted RNA was reverse-transcribed to complementary DNA using RT-qPCR Fast Master Mix (Vazyme, China). .. RT-qPCR was performed on a Article Title: Epigenetic Regulation of Immune Dysfunction in Chronic Prostatitis/Chronic Pelvic Pain Syndrome. Article Snippet: .. Total RNA was extracted from the VB3 cells with TRIzol reagent (Invitrogen) and reverse‐transcribed with qScript cDNA SuperMix (QuantaBio) following the manufacturers' protocols. qRT‐PCR reactions were prepared with PerfeCTa SYBR Green SuperMix (QuantaBio) and run on a Amplification:Article Title: Pulsed Electric Fields as an Effective Tool for Toxoplasma gondii Inactivation. Article Snippet: Thirty milligrams of mouse brain or heart were homogenized in 300 μL of lysis buffer (Promega, Madison, WI, USA) and 37 μL of Proteinase K (Promega, Madison, WI, USA) using plastic hand-pestle rotating plungers in an Eppendorf, and subsequently incubated at 70 ◦C for 30 min. DNA extraction was performed using a Maxwell 16 Lev Blood DNA Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions. .. Amplification and detection of T. gondii were performed using a Article Title: Surveillance on California dairy farms reveals multiple possible sources of H5N1 influenza virus transmission. Article Snippet: Lysis buffer was then added to the pre-aliquoted samples in BSL3 and subsequent nucleic acid extraction was performed in the BSL2 lab (per approved biosafety protocols) using the NucleoMag RNA kit (Machery-Nagel) on an EpMotion 5075 (Eppendorf), with inclusion of a negative extraction control (PBS) in each extraction run. .. Viral detection required both sample replicates to each exhibit amplification (have CT values) using H5 HA-specific primers TATAGARGGAGGATGGCAGG and ACDGCCTCAAAYTGAGTGTT and probe FAM-AGGGGAGTGGKTACGCTGCRGAC- Black hole quencher, with the iTaq Universal Probes One-Step kit (Bio-Rad, Cat. No. 1725141) on a Reverse Transcription:Article Title: Epigenetic Regulation of Immune Dysfunction in Chronic Prostatitis/Chronic Pelvic Pain Syndrome. Article Snippet: .. Total RNA was extracted from the VB3 cells with TRIzol reagent (Invitrogen) and reverse‐transcribed with qScript cDNA SuperMix (QuantaBio) following the manufacturers' protocols. qRT‐PCR reactions were prepared with PerfeCTa SYBR Green SuperMix (QuantaBio) and run on a |
